Superresolution with full-polarized tomographic diffractive microscopy.
نویسندگان
چکیده
Tomographic diffractive microscopy is a three-dimensional imaging technique that reconstructs the permittivity map of the probed sample from its scattered field, measured both in phase and in amplitude. Here, we detail how polarization-resolved measurements permit us to significantly improve the accuracy and the resolution of the reconstructions, compared to the conventional scalar treatments used so far. An isotropic transverse resolution of about 100 nm at a wavelength of 475 nm is demonstrated using this approach.
منابع مشابه
An introduction to diffractive tomographic microscopy
We present the principles of diffractive tomographic microscopy. Contrary to classical transmission microscopy, this technique is based on a coherent, monochromatic, polarized illumination of the specimen, and records the diffracted light in both amplitude and phase using a holographic detection set-up. Combined with multiple illuminations of the specimen, a tomography is performed, and a numer...
متن کاملGeneration of optical vector beams with a diffractive optical element interferometer.
We present a novel approach to generating radially and azimuthally polarized vector beams that utilize an interferometer constructed from two identical diffractive optical elements. The measured polarization properties of four vector beam states and their phase relationships are in good agreement with theoretical expectations. This interferometer is passively phase stable and robust, making it ...
متن کاملHigh-resolution three-dimensional tomographic diffractive microscopy of transparent inorganic and biological samples.
We have developed a tomographic diffractive microscope, equipped with a fluorescence confocal scanner. We measure experimentally the lateral resolution using an edge method and by comparing tomographic images of the same samples with wide-field and laser scanning confocal microscopy images; a scanning electron microscope image serves as a reference. The experimental resolution is shown to be to...
متن کاملTomographic diffractive microscopy with agile illuminations for imaging targets in a noisy background.
Tomographic diffractive microscopy is a marker-free optical digital imaging technique in which three-dimensional samples are reconstructed from a set of holograms recorded under different angles of incidence. We show experimentally that, by processing the holograms with singular value decomposition, it is possible to image objects in a noisy background that are invisible with classical wide-fie...
متن کاملHigh-resolution tomographic diffractive microscopy in reflection configuration.
Tomographic diffractive microscopy (TDM) is a label-free imaging technique that reconstructs the 3D refractive index map of the probed object with an improved resolution compared to confocal microscopy. In this work, we consider a TDM implementation in which the sample is deposited on a reflective substrate. We show that this configuration requires calibration and inversion procedures that acco...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of the Optical Society of America. A, Optics, image science, and vision
دوره 32 2 شماره
صفحات -
تاریخ انتشار 2015